Zoonotic Trichomonas tenax and a new trichomonad species, Trichomonas brixi n. sp., from the oral cavities of dogs and cats
SSUrRNA nucleotide alignment of 76 sequences that has been used for phylogenetic reconstruction - Figure 2 ITS1_5.8_ITS2 nucleotide alignment of 56 sequnces that has been used for phylogenetic reconstruction - Figure 1 Characterization of dog and cat population (TableS1 and Table S2), identification of Trichomonas tenax and Trichomonas brixi by RT PCR and cell cultivation, names of isolated strains.
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Trees based on the ITS1-5.8S rRNA-ITS2 and SSU regions were constructed using the maximum likelihood method in PhyML with the GTR+I+Γ model. The robustness of the trees were evaluated using bootstrapping with 1000 replicates. Bayesian analyses was performed using MrBayes 3.1.6. with the GTR+I+Γ covarion model in the CIPRES Science Gateway (Miller et al. 2010). Four MCMCs were run for 5,000,000 generations, and the first 25% of the samples were discarded as the burn-in. The tree based on the SSU rRNA genes was constructed using PhyML (bootstrapping with 500 replicates) and a Bayesian analysis as described above. Samples were collected in 2012/2013 from 111 dogs and 122 cats from shelters and individual breeders in the Czech Republic. For each animal, demographic data and health status were recorded (age, sex, breed, housing conditions, and dental status), and breeder consent was obtained. "Clinical signs" and "no clinical signs" dental status indicate the presence or absence, respectively, of any clinical signs of gingivitis and periodontal disease (e.g. gingival hyperemia, gingival recession, dental calculus, tooth loss).