Zoonotic potential of Trichomonas tenax, and a new trichomonad species from the oral cavities of dogs and cats
Description
SSUrRNA nucleotide alignment of 76 sequences that has been used for phylogenetic reconstruction - Figure 2 ITS1_5.8_ITS2 nucleotide alignment of 56 sequnces that has been used for phylogenetic reconstruction - Figure 1
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Trees based on the ITS1-5.8S rRNA-ITS2 and SSU regions were constructed using the maximum likelihood method in PhyML with the GTR+I+Γ model. The robustness of the trees were evaluated using bootstrapping with 1000 replicates. Bayesian analyses was performed using MrBayes 3.1.6. with the GTR+I+Γ covarion model in the CIPRES Science Gateway (Miller et al. 2010). Four MCMCs were run for 5,000,000 generations, and the first 25% of the samples were discarded as the burn-in. The tree based on the SSU rRNA genes was constructed using PhyML (bootstrapping with 500 replicates) and a Bayesian analysis as described above.