DLS-data for nanoparticle characterisation of 1,4-DHP and calix[4]arenes

Published: 13 January 2022| Version 1 | DOI: 10.17632/fnk8krrgpy.1


Characterisation of two-component nanoformulations comprised from cationic amphiphilic 1,4-DHP as one component and the appropriate cationic amphiphilic calix[4]arene as another component at mol% ratios of 100/0, 75/25, 50/50, 25/75, and 0/100; starting from single the appropriate calix[4]arene (100/0) in the first sample and single 1,4-DHP (0/100) in the last sample. The obtained results - hydrodynamic diameters of the main population of nanoparticles, polydispersity index and z-potential of nanoparticles formed by 1,4-DHP/calix[4]arene compositions in aqueous medium determined by the DLS method are presented. Dynamic light scattering (DLS) instrument Zetasizer Nano ZSP (Malvern Panalytical Ltd.) with Malvern Instruments Ltd. Software 7.12 was used for evaluation of self-assembling properties of pure cationic 1,4-DHP and calix[4]arene derivatives and characterisation of obtained nanoparticles formed by them in different ratios or individually.


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For the preparation of stock solution appropriate amount of compounds was dissolved in an appropriate amount of methanol. Samples of compounds for dynamic light scattering (DLS) studies were prepared by thin-film hydration method in an aqueous solution at a final concentration of 0.5 mM for total lipid amount. The appropriate amount of stock solutions was used in order to obtain compositions of 1,4-DHP/calix[4]arene at stoichiometric mol% ratios of 100/0, 75/25, 50/50, 25/75, 0/100, after then the organic solvent was removed in vacuo and the residue was dried in high vacuo for 1 h. An appropriate amount of deionised water was added to each flask for the preparation of work solutions. Samples were prepared by sonication using a bath-type sonicator (Cole Parmer Ultrasonic Cleaner 8891CPX (Vernon Hills, IL, USA)). Samples were sonicated for 30 min at 50 ºC. The obtained aqueous solutions of nanoformulations were characterised by DLS measurements using a Zetasizer Nano ZSP (Malvern Panalytical Ltd., Malvern, UK) instrument with Malvern Instruments Ltd Software 7.12.; specifications– medium: water; refractive index: 1.33; viscosity: 0.8872 cP; temperature: 25 ºC; dielectric constant: 78.5; nanoparticles: liposomes; refractive index of materials: 1.60; detection angle: 173º; wavelength: 633 nm. The data were analysed by using the multimodal number distribution software which was included with the instrument for this purpose. The measurements were performed at least three times for each sample.


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Dynamic Light Scattering