RNA-seq profiling of 368T1 murine KP NSCLC cells deleted for AMPK with AMPKa1 add-back subjected to high glucose conditions, Replicate 2

Published: 02-11-2018| Version 1 | DOI: 10.17632/fvy29f2ptz.1
Contributors:
Lillian Eichner,
Reuben Shaw

Description

This dataset was generated for the paper, "Genetic analysis reveals AMPK is required to support tumor growth in murine Kras-dependent lung cancer models." Murine Kras mutant, p53 null (KP) 368T1 NSCLC cells were deleted for AMPK using the CRISPR/Cas9 system and subsequently stably infected with control vector ("KO") or AMPKalpha1 cDNA ("A1") add-back. These cells were subjected to high glucose (25mM, "HG") conditions for 12 or 18 hours, and profiled by RNA-sequencing. Replicate 2 High Glucose (HG) and No Glucose (NG) conditions were generated simultaneously and analyzed together. Analysis of a single dataset was performed using Replicate 1 ("R1"), and both replicates ("R1" and "R2") were analyzed together as indicated.

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