Data supporting development and validation of liquid chromatography tandem mass spectrometry method for the quantitative determination of bile acids in feces

Published: 19 March 2021| Version 1 | DOI: 10.17632/fvy6cz8bgj.1
Armaghan Shafaei,
Joanna Rees,
Claus Christophersen,
Amanda Devine,
David Broadhurst,
Mary C Boyce


The data presented here support the optimization of feces sample extraction for sensitive quantification of bile acids in human feces using LC-MS/MS. The dataset includes: Comparison between recovery of bile acids from (a) dried feces, (b) dried feces spiked with deuterated internal standards prior to drying, and (c) wet feces Short-term stability data for bile acids standard solutions and fecal extracts containing bile acids after 24 h storage at room temperature and at 6°C Data for multiple freeze-thaw cycles on bile acids recovery in both standard solutions and fecal samples Bile acids recovery from cold and warm extractions data