Transcription Induced Domains form the elementary constraining building blocks of the bacterial chromosome

Description

Microscopy images supporting Figure 4 Fig 4b-i Raw images of the panels comparing the positions of one or two parS tags in one or two conditions. Images correspond to phase contrast of the bacteria outline, YFP of the parSpMT1 tag and CFP of the parSP1 tag. Conditions are desc Fig 4j: Raw data for the analysis of the dynamics of the three lacO arrays inserted in the vicinity of the T7 promoter. LacI-YFP foci dynamics was analyzed for 100 time intervals of 1 sec, for each replicate (N = 4 -8) the median MSDα measurement for ~1,000 trajectories of the fluorescently labeled loci was computed. Experiment were performed in the absence of rifampicin upon induction of the T7 TU.

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Raw data were registered with the StackReg plugin of ImageJ. Spot tracking was performed with Particle Tracker from the mosaic suite. MSD were analysed with Trackart (BMC Res Notes. 2014 May 1:7:274.doi: 10.1186/1756-0500-7-274.)

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