CyTOF - MS Twin Study
Mass cytometry data of PBMCs of 57 monozygotic twin pairs discordant for multiple sclerosis. Samples were barcoded in two batches using a restricted combinatorial 9-choose-3 barcoding strategy using anti-CD45 mAbs. In addition to data of unmanipulated cells (surface panel) intracellular cytokine expression was determined in PBMCs of 25 twin pairs after ex vivo activation using phorbol 12-myristate 13-acetate and ionomycin (intracellular cytokine panel).
Steps to reproduce
Mass cytometry data were normalized using five-elements beads (Fluidigm) and manual gating was applied on the convolute to identify live single cells using FlowJo (BD). The sample convolute was debarcoded by applying Boolean gates in FlowJo. Individual debarcoded live single cell samples are stored in this repository and were loaded into R to perform an hyperbolic arcsine transformation, percentile normalization and further downstream analysis (dimensionality reduction, clustering, inter-twin pair quantification, ...). The attached metadata file links the samples (sample_id) to the respective twin pair (pair), diagnosis (disease_state), batch (batch_id) and disease-modifying therapy (DMT).