Single-cell gene expression data of mESC differentiation time-course toward the neuronal lineage.
Published: 27 September 2017| Version 1 | DOI: 10.17632/g2md5gbhz7.1
Contributor:
Patrick StumpfDescription
Single-cell gene expression data generated using 96x96 fluidigm dynamic arrays. Experimental details including steps to reproduce data are included in the associated manuscript. Briefly, mouse embryonic stem cells kept in 2i+LIF conditions for four passages differentiated towards the neuronal lineage in N2B27 medium as described in the literature (Ying et al. 2003 - DOI: 10.1038/nbt780). At 0h, 24h, 48h, 72h, 96h, 120h and 168h individual cells were sorted by FACS based on light-scatter properties and deposited into 96-well plates containing lysis buffer and RT reagents before processing for qPCR using the Biomark HD.
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Institutions
University of Southampton
Categories
Gene Expression, Time Series, Quantitative Polymerase Chain Reaction, Pluripotent Stem Cell Differentiation