Enteric glial cell differentiation in culture

Published: 20 January 2023| Version 1 | DOI: 10.17632/g2rv2g468f.1
Richard Guyer,


These images were taken to test the hypothesis that glial cells isolated from the myenteric ganglia of the enteric nervous system can differentiate into neurons. GFP+ enteric glial cells were isolated via FACS from the myenteric ganglia of Plp1::GFP/ Baf53b::Cre;R26tdT dual-reporter mice bred in our laboratory and placed in culture immediately after sorting. Immunoflouresence imaging of was performed on these cultured cells 1 day and 5 days after plating. We found no evidence of tdTomato+ cells one day after plating, but by 5 days tdTomao+ neurons emerged. This demonstrates the ability of enteric glial cells to generate neurons under the proper conditions.



Massachusetts General Hospital


Enteric Nervous System, Glial Cells