Peel-and-Swiss Roll Biopsy: A Practical Technique for Tissue Sampling in Fragile Blistering Skin Conditions
Description
1. Video demonstration of the peel-and-roll biopsy technique in a representative patient with pemphigus vulgaris, illustrating the atraumatic retrieval and preparation of a detached epidermal sheet for histopathological examination 2. Supplementary Fig 1. Representative tissue section shows a rolled-up, detached horny layer with mounds of parakeratosis, with focal cluster of acantholytic cells, compatible with Staphylococcal scalded skin syndrome (SSSS) (Hematoxylin and eosin, 100x). 3. Supplementary Fig 2. (A) Representative tissue sections from a patient with pemphigus vulgaris, showing “Swiss-roll” configuration at low-power magnification (Hematoxylin and eosin, 100x). (B) Intraepidermal separation with a blister roof and distinct acantholysis can be seen at high power view (Hematoxylin and eosin, 400x).