Datasets from gender specific auditory brainstem responses (ABRs) from Cav2.3+/+, Cav2.3+/- and Cav2.3-/- mice following click and tone burst stimulation

Published: 10 February 2019| Version 2 | DOI: 10.17632/g6ygz2spzx.2
Marco Weiergräber


Cav2.3+/- embryos (kindly provided by Richard j. Miller, The University of Chicago, Chicago, IL) were re-derived with C57BL/6J mice and maintained with random intra-strain mating obtaining all genotypes (Mouse Genome Informatics; MGI Ref. ID J: 66144). For subsequent ABR recordings, Cav2.3+/+ controls, heterozygous Cav2.3+/- and homozygous null mutant Cav2.3-/- mice (58 animals in total, aged 141.29 ± 0.39 days (~ 20 wks)) were used from both age-matched genders with the following characteristics: Males: Cav2.3+/+: n = 9 (♂), weight 32.72 ± 1.80 g; Cav2.3+/-: n = 9 (♂), weight 31.64 ± 1.12 g; Cav2.3-/-: n = 10 (♂), weight 31.10 ± 0.89 g. Females: Cav2.3+/+: n = 9 (♀), weight 25.41 ± 0.57 g; Cav2.3+/-: n = 10 (♀), weight 25.42 ± 1.36 g; Cav2.3-/-: n = 11 (♀), weight 27.00 ± 0.43 g. For recording of monaural bioelectrical auditory potentials, subdermal stainless steel electrodes were inserted at the vertex, axial the pinnae (+ electrode) and ventrolateral of the right pinna (- electrode). The ground electrode was positioned at the hip of the animal. To verify proper electrode positioning / conductivity, impedance measurements of all electrodes (< 5 kΩ) were carried. Note that some recordings also include 2-channel, i.e. binaural measurements. ABR data acquisition was carried out at a sampling rate of 24.4 kHz and signals were bandpass filtered (high pass 300 Hz, low pass 5 kHz) using a 6-pole Butterworth filter. The individual ABR data acquisition time was 25 ms starting with a 5 ms baseline period prior to the individual acoustic stimulus onset (pre ABR baseline) and exceeding the 10 ms ABR section by another 10 ms baseline (post ABR baseline). Two types of acoustic stimuli were applied for ABR recordings in Cav2.3+/+, Cav2.3+/- and Cav2.3-/- mice using the SigGenRZ software (TDT, USA) and applied via the TDT BioSigRZ platform. The first stimulus entity was a click of 100 µs duration, with alternating polarity (switching between condensation and rarefaction). The second stimulus entity was a 4.5 ms tone burst (transient sinusoidal plus) of alternating polarity with Hann envelope rise and fall times of 1.5 ms duration each. (Note that the file header description states an overall duration of 5 ms). The frequency range covers 1 - 42 kHz in 6 kHz steps. All acoustic stimuli were applied 300 times at a rate of 20 Hz for averaging. Sound pressure levels (SPLs) were increased in 5 dB steps for clicks and 10 dB steps for tone bursts, starting from 0 dB up to 90 dB (increasing SPL mode). For tone burst experiments, low SPLs were sometimes excluded to reduce duration of ABR animal experimental. As the raw data file format (arf.) of the TDT system is not freely accessible, data were exported as txt.-files. Header descriptions for both click and tone burst related ABR txt.-files are also attached.


Steps to reproduce

For detailed description of materials and methods please refer to the related "Data in Brief article.


Bundesinstitut fur Arzneimittel und Medizinprodukte


Transgenic Animal, Mouse Phenotyping, Calcium Channels, Animal Auditory System, Auditory Evoked Response