GC-MS analysis of steatotic hepatocyte-like cells

Published: 07-12-2020| Version 2 | DOI: 10.17632/gd5k5psvf4.2
Mandy Drake,
Daniel Tennant


Human pluripotent stem cells were differentiated to hepatocyte-like cells (HLCs) and challenged with a cocktail of lactate, pyruvate and octanoic acid to induce macrovesicular steatosis. Polar metabolites were harvested and analysed by GC-MS. The raw GC-MS MID and batch analyses are listed here.