Dataset for the paper entitled "A genetic map of the response to DNA damage in human cells" by Olivieri M et al. Cell 2020

Published: 7 July 2020| Version 2 | DOI: 10.17632/gfcn2wmrpf.2
Daniel Durocher, Michele Olivieri


We used pooled CRISPR/Cas9 screens in the human RPE1-hTERT p53-/- cell line against 27 genotoxic agents. The Dataset herein make the primary data available. The files are: * Additional genes of interest (discussion and data relating to TMEM2, ESD, USP37, PHF12, BTAF1 and DRAP1) * Supplementary Raw Data: Data used to make all the graphs in the manuscript * Folder "CRISPR screen readcount files". Readcounts for the screens undertaken as part of this study. * Folder "Raw images for immunoblots": uncropped images for all the blots in the manuscript * Folder: "Additional QC analyses of CRISPR screens": Additional analyses that measure the quality of the screens using Presion-Recall curves of essential genes and an estimate of genes that scored as hits due to batch effects. * Folder "Data_Analysis_Code" R markdown file along with data files * File: "Additional Genes of Interests" contains a short discussion of genes we found noteworthy after analysis of our screen data. Version 2 note: this version differs from Version 1 by a single file (FDRPos_31screens.csv) which has a tab error fixed.



Lunenfeld-Tanenbaum Research Institute, University of Toronto


Data Quality, Primary Data