We isolated an anaerobe bacteria from clinical samples, the VITEK 2 ANC Test Kit, which based on the biochemical method could not identify Odoribacter splanchnicus (probably to the lack of database entries with this strain). We identified it by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) Biotyper (Library BDAL-11897-4274 Version 12, Bruker Daltonics, Bremen, Germany) . The isolated strain was identified Odoribacter splanchnicus with a score of 2.110(the credibility is high). Because of the discrepancy between the results of these two analytical methods, 16S rRNA sequencing was adopted for further verification. The BLAST software ( MEGA software version 10) was used to conduct homology analysis of the gene sequences of the isolated strain and those in the GenBanK database. The isolated strain showed a 100% match to the 16S rRNA of Odoribacter splanchnicus (GenBank No.NR_074535.1) , was in the same clade as Odoribacter splanchnicus and belonged to the same species. The mass spectrometry figure of Odoribacter splanchnicus and Phylogenetic tree can help with the accurate identification of this bacterium in the future.