Single-Cell Transcriptomics Analysis of Cardiac Fibroblasts from the Collagen1a2 Lineage Reveals that They Show Endothelial Features
Description
Cardiac fibroblasts (CFs) are a distinct cell type of mesenchymal origin with highly heterogeneous. A subset of CFs undergoing mesenchymal-endothelial transition (MEndoT) and contributes to cardiac repair by revascularizing the damaged myocardium. We used Col1a2CreERT:R26RtdTomato transgenic mice for tracking resident CFs (RCFs) and 10x scRNA-Seq analysis to determine the subgroup of pre-existing CFs involved in MEndoT that respond to transverse aortic constriction (TAC) injury. The cell trajectory assay showed the presence of cluster 1 RCFs showed the phenotype of stem cells, indicates that these clusters are diverse with regard to differentiation and directly contribute to angiogenesis. Sorted CD200+ CFs showed markedly higher expression levels of pro-angiogenesis-related genes and tube formation ability. Our findings indicated that CD200+ cells are the main group of CFs involved in MEndoT and plays a major role in cell differentiation. Our study will aid the development of new CF-targeted therapeutic strategies for treating cardiac hypertrophy.
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Funding
National Natural Science Foundation of China
82000259
National Natural Science Foundation of China
82170264
National Natural Science Foundation of China
82370270
Scientific and Technological Planning Project of Guangzhou City
202102010191
Scientific and Technological Planning Project of Guangzhou City
202201020620
Key Research and Development Program of Guangzhou City
202206010060
the start-up funding from the Guangzhou Women and Children’s Medical Center
3001024