Determination of IgG specific for the diagnosis of food intolerance in athletes

Published: 12 October 2021| Version 2 | DOI: 10.17632/gmmkwh26r9.2
Contributor:
Kristina Malsagova

Description

This study was conducted to study the presence of food intolerance in a group of athletes using a commercial kit of reagents for the semi-quantitative ELISA determination of allergen-specific IgG antibodies in blood plasma. The study included blood plasma samples obtained from athletes provided by the State Research Center Burnasyan Federal Medical Biophysical Center of the Federal Medical Biological Agency of Russia (https://fmbafmbc.ru/en/). The total sample consisted of 44 athletes. Only after the study participants signed an informed consent form, was blood plasma collected. This study was approved by the independent local research ethics committee of the State Research Center Burnasyan Federal Medical Biophysical Center of the Federal Medical Biological Agency of Russia (Protocol No. 40 of 18 November 2020).

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A set of reagents for carrying out research and educational work using the ELISA method (LLC, Immunotex, Russia) (hereinafter referred to as the Set) was used, intended for the semi-quantitative determination of the IgG class antibodies specific for protein-peptide antigens in the blood plasma of the examined patients, contained in food by the method of enzyme-linked immunosorbent assay (ELISA) in order to identify the risk of somato-allergic reactions upon contact with antigens. The assay is based on a non-competitive, indirect, sandwich-type enzyme immunoassay. One Kit is designed for the analysis of the content of specific in three test samples to 30 allergens (file "List of tested antigens"), 5 calibration samples and 1 control sample - a total of 96 determinations. ELISA analysis was performed on a Multiskan FC microplate photometer (Thermo Scientific, USA) using the instructions for the Reagent Kit provided by the manufacturer.

Institutions

Naucno-issledovatel'skij institut biomedicinskoj himii imeni V N Orehovica

Categories

Biochemistry, Enzymology, Immunochemistry

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