Spatiotemporal Analysis of Human Intestinal Development at Single Cell Resolution: Supplementary Data

Published: 4 January 2021| Version 2 | DOI: 10.17632/gncg57p5x9.2
, Neil Ashley, Doran Khamis, Darren Fowler, Edward Morrissey, Chris Cunningham, Paul R.V. Johnson, Hashem Koohy,


Supplementary data for the study "Spatiotemporal Analysis of Human Intestinal Development at Single Cell Resolution" which utilises single cell RNA-sequencing (scRNA-seq) and spatial transcriptomics (ST) to characterise the origins of human intestinal development between 8 and 22 post conceptual weeks. This supplementary data contains analysed sequencing data that was too large for main publications and details data that was used for comparisons of: cell type marker genes; ST varying genes; Transcription factor (TF) module nodes; Human Phenotype Ontology (HPO) disease comparisons across time and cell clusters; cell cluster comparisons and Gene Ontology Analysis. Specific content: A) An excel file with tabs of supplementary data (overview in first tab) including: 1 Sample overview 2 Endothelial Markers 3 Epithelial Markers 4 Fibroblast Markers 5 Immune Markers 6 Muscle Markers 7 Myofibroblast & Mesothelium markers 8 Neural markers 9 Pericyte Markers 10 Secretory epithelium markers 11 Compartment Markers 12 ST Distance varying genes 13 TF Tree Node Markers 14 TF Location Time Compartment 15 HPO Genes Time Varying 16 HPO Genes Cluster Specific 17 S2 Colon vs TI 18 S2 Time Course 19 Stem Cells TI vs Colon 20 Stem cells vs Stem progenitor 21 Stromal 4 differences 22 CITE-Seq Antibody Sequences 23 Neural GO BP 24 Morphogen modules 25 GO Terms ST dist genes B)Images of ST segments (n=8 tissue segments with low resolution and high resolution images of each)


Steps to reproduce

See related publication for methods


University of Oxford


Genomics, RNA, Spatial Analysis