Artemisia annua Data Zambia by J Ngwisha et al

Published: 4 September 2024| Version 1 | DOI: 10.17632/gsk34rzb3p.1
Contributors:
Joshua Ngwisha,
,
,
,

Description

This study evaluated the antimicrobial activity and phytochemical profile of Artemisia annua extracts collected from Zambia, highlighting potential applications in combating Antimicrobial resistance. Utilizing disk diffusion and broth microdilution methods, we assessed the efficacy of various solvent extracts against a range of bacterial strains, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus agalactiae. Our findings reveal significant activity against a spectrum of bacteria, with the ethanol extract displaying superior efficacy. These results advocate for Artemisia annua as a promising candidate in the development of new antibacterial agents.

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Steps to reproduce

Plants were collected following a non-participatory rapid rural appraisal. Herbs were air-dried. Sequential Exhaustive Extraction was used for maceration. Characterized bacterial pathogens were used in the study. Disk diffusion was used to pilot and triangulate the study, broth microdilution was used for the main assays. Optical density was measured pre and post-incubation. Glassware used included test tubes, conical flasks, evaporation flasks, measuring cylinders, Petri dishes, and microtiter plates. Other equipment included a sonicator, drying oven, rotary evaporator. Data collation was in MS excel. Analysis in Statistical Analysis Software version 9.4.

Institutions

University of Zambia School of Veterinary Medicine

Categories

Antimicrobial Resistance, Plant Natural Product, Phytopharmaceutical

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