VitaminDMetabolomics_Knuthetal2024_Frontiers
Description
The effect of developmental vitamin D deficiency on adult liver metabolite profiles was determined. Untargeted profiling of whole liver metabolites from adult male Collaborative Cross mice was performed using mass spectrometry (Metabolon, Inc.).
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Four platforms were utilized by Metabolon, Inc. in order to capture metabolites across a wide range of varying chemical properties: 1) RP/UPLC-MS/MS with acidic positive ion mode electrospray ionization (ESI) for hydrophilic compounds, 2) RP/UPLC-MS/MS with acidic positive ion mode ESI for hydrophobic compounds, 3) RP/UPLC- MS/MS with basic negative ion mode ESI, and 4) HILIC/UPLC-MS/MS with negative ion mode ESI. Metabolites were identified based on spectrum properties (m/z and retention time/index). All raw data were median normalized and samples with measurements that fell below the limit of quantitation were imputed to the limit of quantitation. Sample demographics are as follows: Mus musculus; Male; F1 Collaborative Cross offspring; 8-9 weeks of age; exposed to a vitamin D sufficient (CON) or vitamin D deficient (LVD) diet from conception until weaning. Strains include: 1b (CC001-dam x CC011-sire) and 1a (CC011-dam x CC001-sire).