(supplementary data) Development and Characterization of pH-sensitive Zerumbone-encapsulated Liposomes for Lung Fibrosis via Inhalation Route
Description
Context and Purpose: This dataset provides supplementary material for the manuscript titled "Development and Characterization of pH-sensitive Zerumbone-encapsulated Liposomes for Lung Fibrosis via Inhalation Route." The data support the findings and analyses presented in the main manuscript, offering additional details on experimental procedures and results.
Files
Steps to reproduce
Zerumbone Liposome Preparation 1. Add 7 mg of the liposomal composition of DPPC, Cholesterol, Oleic acid, and Zerumbone at 58:17:11:14 (%w/w) to a sealed glass tube. Then solubilize them in 1000 µL pure ethanol. 2. In the fume hood, dry the mixture under a gentle stream of nitrogen. 3. To the dried-down mixture, add 3 ml room temperature PBS solution and cover the end of the glass tube with the cap and vigorously shake it for 2 minutes to completely resuspend the mixture. The result should be a milky, uniform suspension. 4. Fill the bath sonicator with distilled water. Place the sample in the middle of the Elmasonic S 60H Ultrasonic device. The liquid level inside and outside of the tube should be the same and the temperature should be adjusted to 50 °C before placing the sample. Sonicate the suspension until it turns from milky to a little bit hazy. Check every 10 minutes; it will usually take approximately 30 minutes of total sonication time. 5. Store the final product at 4 degrees Celsius. The result is a suspension of small unilamellar vesicles (SUVs).
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Funding
Taylor’s University
TAYLOR'S RESEARCH SCHOLARSHIP Programme.
Taylor’s University
My CytoHealth Sdn. Bhd., Taylor’s University
Monash University Malaysia
CYTOHEALTH/2023/SOP/002 and P-M010-MTC-000150