Using membrane acting-agents and ultrasound to overcome biological barriers and improve treatment of a methicillin-resistant Staphylococcus aureus diabetic wound infection. Papadopoulou et al.
Wound infections respond poorly to antibiotic therapy due to poor drug penetration and the presence of persister cells within biofilms. This study targets both of these obstacles by combining a novel aminoglycoside adjuvant with potent anti-persister activity with Sonotherapy. Sonotherapy is an ultrasound-mediated method to mechanically improve drug delivery. Palmitoleic acid, a novel aminoglycoside adjuvant, sensitized multiple gram positive pathogens to gentamicin. Combining this drug combination with Sonotherapy in vivo, improved treatment of a methicillin-resistant wound infection in diabetic mice.. Together, this data suggests that using a combined approach to target persister cells and improve drug penetration is a promising strategy to combatting recalcitrant wound infections. This data contains a prism file containing in vitro data (Fig. 1-4) and a separate prism file containing the in vivo data from the study (Fig. 5-6).