Multiplex plasma cytokine and PBMC flow cytometry analysis long-term COVID-19 patients

Published: 8 May 2023| Version 1 | DOI: 10.17632/gxmnms9whf.1
Elena Montes Cobos,
Victoria Bastos,
Clarice Monteiro,
João Carlos Ramalho,
Heiny Fernandes,
Clarice Constancio,
Danielle Rodriguez,
Andreza Gamma,
Vinicius Mendes Vidal,
Leticia Alves,
Laura Zalcberg-Renault,
Guilherme de Lira,
Victor Akira Ota,
Luciana Conde,
Andre Macedo Vale,
Juliana Echevarria,
Marcelo Torres Bozza


Our aim was to compare the immune responses of mildly-symptomatic COVID-19 patients with regular course of infection to those of patients with persistent SARS-CoV-2 infection. These set of data was obtained from plasma and PBMCs purified from healthy human individuals (HC) and COVID-19 patients with either a short infection period (until 21 days after symptom onset, NP) or with long-term infection (detectable SARS-CoV-2 RNA by qRT-PCR in nasopharynx for longer than 21 days after symptoms onset, P). The data represent a set of plasma cytokines and flow cytometry imunophenotyping data obtained from frozen plasma and PBMCs from the refered study subjects. We observed significant diferents in several of the analyzed parameters between the study groups, indicating distinct immune responses defining the group of olygosymptomatic long-term infected patients.



Universidade Federal do Rio de Janeiro


Cytokines, Flow Cytometry