MUC1, MUC4, Stn, TN dataset in cervical cancer
Description
The photomicrographs are images of immunohistochemically stained cervical cancer and lymph node metastases. The specimens were prospectively collected from patients enrolled in the Gynecologic Oncology Group (GOG) clinical trial GOG0221: Glycoprotein and glycan profiling in patients with locally advanced cervical cancer (Stage IB2, IIA > 4 cm, IIB to IVA) undergoing pelvic and para-aortic (abdominal) lymphadenectomy (clinicaltrials.gov:NCT00460356). Sections of the specimens immunohistochemically stained for mucin 1 and 4 proteins (MUC1 and MUC4, respectively) and Sialyl Tn (STn) and Tn antigens were reviewed by experienced gynecologic pathologists and areas of interest for photomicrography were selected by the first two authors of this brief (R.R. and R.C.). Representative images for all immunohistochemical stains showing their expression patterns as well as positive and negative controls were shown and described.
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Prospectively collected and banked specimens from the clinical trial GOG 0221 (NCT00460356) were obtained with permission from the National Research Group (NRG) Oncology and National Cancer Institute (NCI). These formalin-fixed, paraffin-embedded specimens were derived from 139 patients >18 yrs of age with previously untreated stage IB-IVA (AJCC TNM staging system, 7th edition) primary cervical cancer, who underwent local surgical control with possible lymphadenectomy. The total number of specimens are as follows: 133 primary cervical cancer specimens, 28 para-aortic lymph node specimens, and 57 pelvic lymph node specimens (including the obturator, external iliac, and common iliac lymph nodes). Sections from each specimen block underwent immunohistochemical staining using an automated tissue immunostainer (Leica Bond-III Polymer Refine Detection System DS9800, Leica Biosystems, Deer Park, IL, USA) and the following primary antibodies: Tn (1:200 undiluted tissue culture supernatant, clone 5F4, mouse mAb IgM, H. Clausen / U. Mandel, Copenhagen Center for Glycomics, University of Copenhagen, Denmark), Sialyl Tn (1:200 undiluted tissue culture supernatant, clone TKH2, mouse mAb IgG1, H. Clausen / U. Mandel, Copenhagen Center for Glycomics, University of Copenhagen, Denmark), MUC1 (dilution 1:100, clone HPA004279, rabbit pAb, Sigma-Aldrich, Inc., St. Louis, MO, USA), and MUC4 (dilution 1:400, clone HPA005895, rabbit pAb, Sigma-Aldrich, Inc., St. Louis, MO, USA). Positive validation of biomarker staining was performed on sections of murine colon from T-synthase knockout mice (villin::Cre/floxed C1GalT1, Lijun Xia, Oklahoma Medical Research Foundation, Oklahoma City, OK); negative validation was performed on sections of murine colon from C57Bl/6J wild-type mice. Evaluation of stained slides was performed by experienced gynecologic pathologists who were completely blinded to clinical parameters. The H-scores are provided in the companion Gynecologic Oncology article. To collate various tumor staining characteristics, an experienced gynecologic pathologist selected and photographed pertinent cases using a Nikon Eclipse Ni microscope (Minato City, Tokyo, Japan) utilizing NIS-Elements AR 3.2 64-bit software.