Diffusion tensor microscopy data (15.6um in-plane) of white matter tracts in the human, pig, and rat spinal cord with corresponding tissue histology

Published: 29-09-2016| Version 1 | DOI: 10.17632/h3wkngnptj.1
Jeremy Flint,
Brian Hansen,
Stephen Blackband


This data consists of nine diffusion tensor imaging (DTI) datasets acquired with magnetic resonance microscopy (MRM, 15.6 um in-plane) as well as the corresponding histology for each of the nine samples. Cross sections of fixed spinal cord from human (6), rat (2) and pig (1) sources were imaged along the boundary of gray and white matter in the ventral horn. Following MR collections, the samples were stained using Nissl or Black Gold II to label alpha-motor neuron cell bodies and myelin respectively. After staining, a second series of images were obtained using bright-field microscopy techniques. Thus, the corresponding histology images can be used as a reference for the microscopic structures (cell bodies, axon bundles) visualized by the MR microscopy data.