Digestion of recombinant O-GlcNAcylated human tau with different enzymes - RAW data files - nLC-Q Exactive HF acquisition
Published: 12 December 2022| Version 1 | DOI: 10.17632/h5vdrx4n3d.1
O-GlcNAcylated recombinant human tau was digested with different enzymes, rendering 5 digestion conditions: trypsin only (1), asp-N only (2), glu-C only (3), trypsin followed by asp-N (4) and trypsin followed by glu-C (5). nLC-HRMS analysis was performed for the identification of tau O-GlcNAc sites. Data were acquired in data dependent MS2 (ddMS2) mode and for low abundant O-GlcNAc peptides also in parallel reaction monitoring (PRM) mode.
Post-Translational Modification, Liquid Chromatography Mass Spectrometry, Tauopathy