Additional SI item

Published: 17-10-2018| Version 1 | DOI: 10.17632/h72f6mf6z9.1
Contributors:
Ryu Yamanaka,
Yutaka Shindo,
Kohji Hotta,
Koji Suzuki,
Kotaro Oka

Description

Additional SI item. Scheme of neurite and synapse quantification. Related to Figure 4. (A) Representative image of Tuj-1 immunofluorescence and DAPI staining. (B) Binary neuron image converted from the Tuj-1 fluorescence image by thresholding. (C) Binary nuclear image converted from the DAPI fluorescence image by thresholding. (D) Magnified views of the boxed area in panel A. (E) Merged image of the original and segmented neurites. (F) Binary image of neurons that was skeletonized to obtain a neurite image. (G) Schematic of the segmentation of the synapse adjoining neurons; neuron and synapse were segmented from cytosol-expressing mCherry and synapsin fluorescence image. Synapses were selected by synapse puncta adjoining neurons. (H) Fluorescence image of neurons expressing mCherry. (I) Fluorescence image of the synapse marker synapsin. (J) Merged image of mCherry and synapsin fluorescence. (K) Merged image of original and segmented neurites (blue) and synapses adjoining neurons (black dots with a yellow outline). Scale bars: 200 μm (A), 40 μm (D) and 50 μm (H).

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