Integrated single-cell transcriptomic and network analysis identifies key regulators of Acinetobacter baumannii biofilm integrity

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Biofilm growth curves of Acinetobacter baumannii were determined using the crystal violet staining method. Gene expression differences among different cell clusters were compared based on single-cell RNA sequencing data and validated by RT-qPCR. Functional validation of the target gene was further performed by comparing biofilm formation between wild-type (WT) and gene-knockout (KO) strains. Compared to the WT strain, the KO strain exhibited significantly reduced biofilm biomass and permeability, confirming the gene's role as a key regulator in biofilm formation. Targeting this gene may disrupt biofilm-associated drug resistance, providing a novel strategy for treating persistent infections.

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