Modulation of autophagy in GR-HepG2 cells by Citrus flavonoids from bergamot: flow cytometry qualitative analysis of autophagy index

Published: 29 May 2018| Version 1 | DOI: 10.17632/h76zkv7zjh.1
Contributors:
Elzbieta Janda,
Antonella Lascala,
Concetta Martino

Description

Bergamot Polyphenol Fraction (BPF) is a natural mixture of Citrus flavonoids extracted from processed bergamot fruits. It has been shown to counteract cardiovascular risk factors and to prevent liver steatosis in rats and patients. Hepatic effects of BPF correlate with its ability to stimulate liver autophagy. Six aglyconic flavonoids have been identified in the proautophagic fraction of the hydrolysis product of BPF (A-BPF): naringenin, hesperetin, eridictyol, diosmetin, apigenin and luteolin. This set of data addresses the effects of the six flavonoids on autophagy in HepG2 cells. The Excel file contains tables with mean fluorescence intensities values recorded in three independent experimental settings (each time in triplicate independent cell samples, in over 72 independent cell samples in total). This Excel file also shows how mean ATG index is calculated and normalized and performs statistical analysis of the data. Examples of flow cytometry dot-plots with raw data and relevant gates used for analysis are also attached. These raw data support the data presented in Fig. 4D of the manuscript published in Journal of Nutritional Biochemistry entitled “Analysis of proautophagic activities of Citrus flavonoids in liver cells reveals the superiority of a natural polyphenol mixture over pure flavones” by Lascala et al. For further information see: https://doi.org/10.1016/j.jnutbio.2018.04.005. .

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Institutions

Universita degli Studi Magna Graecia di Catanzaro

Categories

Pharmacology, Cell Biology, Autophagy, Flow Cytometry, Natural Compound

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