Gas chromatography-mass spectrometry separation method of 2-furanyl fentanyl and 3-furanyl fentanyl

Published: 26-02-2019| Version 1 | DOI: 10.17632/h943vhrzf5.1
Contributors:
Olivia Duong,
Steven Composto,
John Gilligan,
Vito Casella,
Matthew Johnson

Description

A GC-MS method was developed to separate the positional isomers of 2-furanyl fentanyl and 3-furanyl fentanyl. This method is useful to forensic laboratories with limited resources for the routine qualitative detection of either 2- or 3-furanyl fentanyl isomer. The method utilizes a 100% dimethylpolysiloxane stationary phase, 30m x 0.25mm x 0.25µm column with pulsed splitless injection and is able to achieve a resolution of 1.78. Forensic evidentiary samples were run in tandem with samples of the isomeric mixture in which 2-furanyl fentanyl was identified via retention time and mass spectral data. The data files are CSV files of total ion chromatogram data.

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Steps to reproduce

All samples were analyzed using GC-MS (Agilent 6890N gas chromatograph fitted with a 7683 autosampler and coupled to an Agilent 5973 mass selective detector) with Agilent Chemstation Data Analysis software (version E.02.02). The optimized GC parameters were: column: Rxi-1ms (length: 30 m, I.D.: 0.25 mm, film thickness: 0.25 μm); pulsed splitless injection; injection port temperature: 280 °C; carrier gas: helium; flow rate: 1.5 mL/min; oven temperature: initially 100 °C for 1.0 min, ramped to 280 °C at 12 °C/min, hold final temperature for 9.0 min (total run time 25 min). The MS conditions were: transfer line heater: 280 °C; MS source temperature: 230 °C; MS quadrupole temperature: 150 °C. Analysis was performed in scan mode, from m/z 35 to m/z 500 and the solvent delay was set to 2 min.