Gas chromatography-mass spectrometry separation method of 2-furanyl fentanyl and 3-furanyl fentanyl

Published: 26 February 2019| Version 1 | DOI: 10.17632/h943vhrzf5.1
Olivia Duong, Steven Composto, John Gilligan, Vito Casella, Matthew Johnson


A GC-MS method was developed to separate the positional isomers of 2-furanyl fentanyl and 3-furanyl fentanyl. This method is useful to forensic laboratories with limited resources for the routine qualitative detection of either 2- or 3-furanyl fentanyl isomer. The method utilizes a 100% dimethylpolysiloxane stationary phase, 30m x 0.25mm x 0.25µm column with pulsed splitless injection and is able to achieve a resolution of 1.78. Forensic evidentiary samples were run in tandem with samples of the isomeric mixture in which 2-furanyl fentanyl was identified via retention time and mass spectral data. The data files are CSV files of total ion chromatogram data.


Steps to reproduce

All samples were analyzed using GC-MS (Agilent 6890N gas chromatograph fitted with a 7683 autosampler and coupled to an Agilent 5973 mass selective detector) with Agilent Chemstation Data Analysis software (version E.02.02). The optimized GC parameters were: column: Rxi-1ms (length: 30 m, I.D.: 0.25 mm, film thickness: 0.25 μm); pulsed splitless injection; injection port temperature: 280 °C; carrier gas: helium; flow rate: 1.5 mL/min; oven temperature: initially 100 °C for 1.0 min, ramped to 280 °C at 12 °C/min, hold final temperature for 9.0 min (total run time 25 min). The MS conditions were: transfer line heater: 280 °C; MS source temperature: 230 °C; MS quadrupole temperature: 150 °C. Analysis was performed in scan mode, from m/z 35 to m/z 500 and the solvent delay was set to 2 min.


Analytical Chemistry, Opioid, Gas Chromatography Mass Spectrometry, Forensic Chemistry