Effects of Rhizosphere Nitrogen-fixing, Phosphate-solubilizing and Potassium-solubilizing Bacteria on Leaf Nutrients and Physiological Traits in Different Natural Populations of Malus sieversii (Ldb.) Roem.
Description
ANOVA and correlation analysis showed that the impact of NFB, PSB, and KSB colonization numbers on physiological traits and to determine the number of rhizosphere microorganisms when wild apples grow better in Xinjiang for their healthy growth.
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Sampling sites in Xinjiang, China (Daxigou in Huocheng County, Wild Fruit Forest in Xinyuan County, Resource Nursery, Damorhu in Gongliu County, Xiaomorhu in Gongliu County, and Gongliu County Nazi Work Team, Laofengkou Guozigou in Tuoli County, and Yeguolin Scenic Area in Emin County) are summarized in the following table (Table 1). Wild apple rhizosphere soil sampling was carried out in May 2021. Randomly selected trees with a diameter at breast height of 90-100 cm were sampled, the topsoil was removed with an autoclaved spade, and the fibrous roots were located along the lateral roots in the 10-20 cm soil layer; the roots were dug out, placed in sterile bags and brought back to the laboratory for storage at 4°C. Three replicates of each population were sampled and each replicate was mixed by multi-point sampling, a total of 24 samples were collected. Leaves were collected in July, placed in a holding tank and taken back to the laboratory for storage at -80°C for use in subsequent experiments.