A food-grade vector for Streptococcus thermophilus based on the α-complementation of β-galactosidase

Published: 5 May 2022| Version 1 | DOI: 10.17632/hb7d2dh9ys.1
Zhenshang Xu,
Jian Kong,
Xinli Liu,


Supplementary Table 1 Strains and plasmids used in this study; Supplementary Table 2 Oligonucleotides used in this work; Supplementary Table 3 Growth of recombinant strains of E. coli and S. thermophilus in lactose medium; Supplementary Table 4 Physicochemical properties of yogurt by different inoculation methods; Supplementary Figure 1 Sequence alignment of β-galactosidases in E. coli and S. thermophilus by the online available program PAIRWISE SEQUENCE ALIGNMENT; Supplementary Figure 2 The stability of vector pSEα in S. thermophilus α grown in LM17 medium. Error bars represent standard deviation (SD) from 3 replicate experiments.



Qilu University of Technology


Dairy Microbiology