SEM-EDS Bacteria image library
In-Vitro Pb Uptake by Bacteria in Liquid Cultures-Scanning Electron Microscopy (SEM-EDX) Analysis. B. paramycoides, B. proteolityticus and B. wiedmannii were able to immobilise Pb on their cell wall.
Steps to reproduce
Batch studies were conducted using 50 mL Falcon tubes that contained 25 mL GY rich medium supplemented with 100 mg L-1 of Pb (Pb(NO3)2). All tubes were inoculated with 1 mL of a bacterial cell suspension (optical density at 600 nm=1), which was previously grown in GY medium at 28°C for 24 h. Samples were incubated at 28°C and 100 rpm on an orbital shaker. For the visualization of bacterial structure, bacterial pellets were washed 3 times with 0.01 M phosphate-buffered saline buffer (PBS, pH 7.0) to remove unbound metals, sugars and proteins. Pellets were resuspended in 2% glutaraldehyde for 1h at room temperature. Afterwards, bacterial samples were centrifuged for 3 min at 3000 rpm and pellets were washed 3 times with milli-Q water. One uL of sample was placed on a sample holder, in carbon conductive tape. Then, samples were coated 30 seg with a 15 nm gold layer and analysed using a Scanning Electron Microscope (Phenom™ ProX Desktop, Thermofisher) with an integrated energy-dispersive X-ray diffraction (EDS) detector. Images were taken using an accelerating voltage of 15 kV.
BOF 2022 – Bilateral Scientific Cooperation (R-12630)