Data for: Isolation and characterization of Acidithiobacillus caldus TST3 and the effect of pre-cultivation on its dissolution of phosphorous from rock phosphates

Published: 22 March 2018| Version 1 | DOI: 10.17632/hcv8swtpht.1
Lingling li, Xiaorong Qi, Zaosheng Lv, Zhong-Hua Yang, Yaoning Liu, Haiyan Guan, WenTing Zhou, Zhenyu Zuo


we report the results of the first study on isolation and characterization of Acidithiobacillus caldus TST3 and the effects of pre-cultivation on improving the bio-leaching of rock phosphates by this microorganism. Indeed, we isolated and identified Acidithiobacillus caldus TST3, studied the dissolution of rock phosphates by strain TST3 in a series of bio-leaching experiments with and without pre-incubation, and then elucidated the relevant mechanism involved in the enhanced bio-solubilization. The results from our study reveal that Acidithiobacillus caldus TST3 has some unusual physiological characteristics with optimal growth at 37ºC and at an initial pH of 1.5-2.0. No pre-cultivation was observed to enhance bio-leaching significantly in comparison with that achieved with pre-grown culture in a phosphorous-limited environment. We presumed that the reason for the enhanced bio-leaching without pre-cultivation may results from the enhancement of the expression of sulfur oxidation genes, bio-oxidative activity of elemental sulfur and bacterial growth due to the simultaneous addition of RPs with inoculation.