Epoxy-tiglianes induce skin keratinocyte wound healing responses and re-epithelialization via classical protein kinase C activation (Supplementary Data)
Description
Epoxy-tiglianes are a novel class of diterpene esters isolated from a native Australian rainforest plant, Fontainea picrosperma (Euphorbiaceae). The prototype epoxy-tigliane, EBC-46 (tigilanol tiglate), possesses potent anti-cancer properties and is currently under clinical evaluation as a veterinary and human cancer treatment for cutaneous and subcutaneous tumors, in part mediated via the activation of classical protein kinase C (PKC) isoforms. EBC-46 also stimulates exceptional dermal wound healing in vivo upon tumor destruction, manifested as accelerated wound re-epithelialization and closure. We have previously shown that epoxy-tiglianes stimulate proliferative and wound repopulation responses in immortalized human skin keratinocytes (HaCaTs) in vitro; and identified the key gene and protein targets modulated which facilitate such enhanced wound healing response. The data presented provides supplementary information to our manuscript, identifying the specific PKC isoforms responsible for inducing proliferative and migratory responses in human keratinocytes, following treatment with EBC-46 or analogue, EBC-211. Specifically, the data shows the effects of specific classical PKC (GӦ6976), PKC-α (Ro 31-8220 mesylate) and PKC-βI/-βII (enzastaurin) isoform inhibitors on the enhanced scratch wound repopulation induced by EBC-46 and EBC-211 in vitro (automated time-lapse Confocal Microscopy movies). Additionally, although the effects of classical PKC (GӦ6976) and PKC-βI/-βII (enzastaurin) isoform inhibitors on the differential expression of specific genes of interest shown to be significantly different at the protein level are included in the main manuscript (by Western blotting and ImageJ densitometry), non-significant differences in Western blot protein profiles between untreated and epoxy-tigliane-treated keratinocytes, +/- these PKC inhibitors, are shown here.
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Qbiotics