Molecular and cellular effect of Achilles tenotomy and tendon reconstruction on rat soleus muscle

Published: 21-06-2018| Version 1 | DOI: 10.17632/hdmgydpr3v.1
Contributors:
Martin Flück,
Céline Ferrié,
Stephanie Kasper

Description

animal code and experimental assigment of the measured volume, cellular, and molecular data

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Achilles tenotomy, tendon reattachment and transfection of soleus muscle with expression plasmid in Wistar rats Cryo-preservation and histological analysis of fiber type composition of rat soleus muscle Immunohistochemistry/fluorescence of FAK expression Biochemical analysis of FAK, JNK; mTOR and p70S6K protein content and phosphorylation via immunoblotting and electrochemiluminescence-based immunoassay