Molecular and cellular effect of Achilles tenotomy and tendon reconstruction on rat soleus muscle
Published: 21 June 2018| Version 1 | DOI: 10.17632/hdmgydpr3v.1
Contributors:
Martin Flück, , Description
animal code and experimental assigment of the measured volume, cellular, and molecular data
Files
Steps to reproduce
Achilles tenotomy, tendon reattachment and transfection of soleus muscle with expression plasmid in Wistar rats Cryo-preservation and histological analysis of fiber type composition of rat soleus muscle Immunohistochemistry/fluorescence of FAK expression Biochemical analysis of FAK, JNK; mTOR and p70S6K protein content and phosphorylation via immunoblotting and electrochemiluminescence-based immunoassay
Institutions
Universitat Zurich
Categories
Anatomy, Focal Adhesion Kinase, Muscle, Atrophy, Muscle Contraction