Data on chemical and pharmacological characteristics of simplicia and ethanol extract of Moringa oleifera leaves
Description
The chemical and pharmacological characteristics of simplicia and ethanol extract of Moringa oleifera leaves data consisting of water content, acid-insoluble ash content, microbial contamination, heavy metal contamination, phytochemical analysis, TLC analysis, chemical profiling using LC-MS/MS, and the antioxidant activity. The data obtained on the characteristics of M. oleifera leaves simplicia were compared with the standards set by the Indonesian Herbal Pharmacopoeia. Qualitative phytochemistry indicates that the ethanol extract of M. oleifera leaves contains flavonoids, tannins, and steroids. In total of 39 phytoconstituents were tentatively identified; the top 10 active compounds with the highest relative abundance percentage (%) are 4-undecylbenzenesulfonic acid (4,83), apigetrin (3,34), quercetin-3β-D-glucoside (3,28), D-(-)-quinic acid (1,69), corchorifatty acid F (1,52), 4-hydroxybenzaldehyde (1,47), isopropylmalic acid (1,17), 13(S)-HOTrE (1,08), astragalin (0,99), and D-(+)-phenyllactic acid (0,70). The ethanol extract of M. oleifera leaves contained a total phenolic content 7728,02 mg/kg, total flavonoids as quercetin content 1,19%, and antioxidant activity IC50 1422,45 mg/kg.
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M. oleifera leaves collected in Kokalukuna district, Baubau city, Southeast Sulawesi. Geographical coordinates: 05o 25’41.68” S 122o 39’12.47” E or 51M 461611 939987. M. oleifera leaves simplicia was tested for water content, ash content, and acid-insoluble ash content using gravimetric analysis techniques, while microbial contamination and metal contamination were analyzed using the pour plate method and Inductively Coupled Plasma – Optical Emission Spectrometry (ICP OES), respectively. Qualitative phytochemical data for the ethanol extract of M. oleifera leaves were obtained through color visualization. The UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS Thermo Scientific method was used to identify the active compounds. The total phenolic and total flavonoid as quercetin content in the ethanol extract of M. oleifera leaves were tested using UV-vis spectrophotometer, while antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method.
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Center for Education Financial Services, Beasiswa Pendidikan Indonesia (BPI), Ministry of Education of Republic of Indonesia collaborate with LPDP (the Indonesian Endowment Fund for Education, Ministry of Finance of Republic of Indonesia)