Single cell RNA-seq of human epidermis

Published: 19-07-2021| Version 1 | DOI: 10.17632/hjms342gt6.1
Contributor:
Zhikai Wang

Description

single-cell RNA sequencing (scRNA-seq) on the epidermis derived from healthy donors and psoriasis patients

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Steps to reproduce

Fresh skin was placed in saline at 4°C until further processing. Single cell suspensions were generated by enzyme digestion and subjected to FACS to exclude doublets, debris and DAPI-positive dead cells. Sorted cells were centrifuged and resuspended in 0.04% BSA in PBS. Chromium Single Cell 3’ v3 (10x Genomics) library preparation was conducted by the Sequencing Core at the Shanghai Institute of Immunology, according to the manufacturer’s instructions. The resulting libraries were sequenced with an Illumina HiSeq 4000 platform. Raw data were processed using Cell Ranger (version 3.0) and further filtered, processed and analyzed using the Seurat package.