Individual genotyping of caged red foxes from the analysis of 14 microsatellite targets and one sex marker

Description

Description of the dataset Individual genotyping of caged red foxes (H for hair, F for faeces) from the analysis of 14 microsatellite targets (AHT121, AHT137, C01.424, INU055, AHTh171, C08.618, CPH2, FH2010, C04.140, CXX0279, FH2848, REN169O18, CPH11, FH2457) and one sex marker (K9-AMELO) (Breen et al., 2001; Fredholm and Winterø, 1995; Holmes et al., 1995; Ichikawa et al., 2002; Moore et al., 2010; Ostrander et al., 1993) ND for no data. The experimental station of the French National Reference Laboratory (NRL) at the ANSES laboratory (Atton, Meurthe-et-Moselle, France) provided 20 hair samples and 20 faecal samples taken in pairs from caged red foxes (n = 20; 10 females, 10 males). The faecal samples were collected shortly after deposition in the animal cages. To avoid cross-contamination, all samples were placed separately in individual sealed bags and frozen at – 20°C until laboratory analyses. Genomic DNA was extracted from hair root using the Qiagen DNeasy tissue kit (Qiagen, Hilden, Germany) following the manufacturer’s recommendations. The QIAmp Fast DNA Stool kit (Qiagen, Hilden, Germany) was used to extract genomic DNA from faecal samples. Fragment size analyses were performed to obtained allele size quantification for the 14 microsatellites and the sex marker, by using a SeqStudio Genetic Analyzer (Applied Biosystems, Foster City, CA). Genetic profiles were determined using the Microsatellite Analysis module available on the Thermo Fisher cloud (https://apps.thermofisher.com/editor-web/#/app/app-microsatellites-web). References Breen, M., Jouquand, S., Renier, C., Mellersh, C.S., Hitte, C., Holmes, N.G., Chéron, A., Suter, N., Vignaux, F., Bristow, A.E., Priat, C., McCann, E., André, C., Boundy, S., Gitsham, P., Thomas, R., Bridge, W.L., Spriggs, H.F., Ryder, E.J., Curson, A., Sampson, J., Ostrander, E.A., Binns, M.M., Galibert, F., 2001. Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes. Genome Res. 11, 1784–1795. Fredholm, M., Winterø, A.K., 1995. Variation of short tandem repeats within and between species belonging to the Canidae family. Mamm. Genome Off. J. Int. Mamm. Genome Soc. 6, 11–18. Holmes, N.G., Dickens, H.F., Parker, H.L., Binns, M.M., Mellersh, C.S., Sampson, J., 1995. Eighteen canine microsatellites. Anim. Genet. 26, 132–133. Ichikawa, Y., Takahashi, Y., Tsumagari, S., Takeishi, M., Ishihama, K., Morita, M., Kanemaki, M., Minezawa, M., Takahashi, H., 2002. Identification and characterization of 40 dinucleotide microsatellites in the dog genome. Anim. Genet. 33, 400–401. Moore, M., Brown, K.S., Sacks, A., 2010. Thirty-one short red fox (Vulpes vulpes) microsatellite markers. Mol. Ecol. Resour. 10, 404–408. Ostrander, E.A., Sprague, G.F., Rine, J., 1993. Identification and characterization of dinucleotide repeat (CA)n markers for genetic mapping in dog. Genomics 16, 207–213.

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Individual genotyping of caged red foxes (H for hair, F for faeces) from the analysis of 14 microsatellite targets (AHT121, AHT137, C01.424, INU055, AHTh171, C08.618, CPH2, FH2010, C04.140, CXX0279, FH2848, REN169O18, CPH11, FH2457) and one sex marker (K9-AMELO) (Breen et al., 2001; Fredholm and Winterø, 1995; Holmes et al., 1995; Ichikawa et al., 2002; Moore et al., 2010; Ostrander et al., 1993) ND for no data. The experimental station of the French National Reference Laboratory (NRL) at the ANSES laboratory (Atton, Meurthe-et-Moselle, France) provided 20 hair samples and 20 faecal samples taken in pairs from caged red foxes (n = 20; 10 females, 10 males). The faecal samples were collected shortly after deposition in the animal cages. To avoid cross-contamination, all samples were placed separately in individual sealed bags and frozen at – 20°C until laboratory analyses. Genomic DNA was extracted from hair root using the Qiagen DNeasy tissue kit (Qiagen, Hilden, Germany) following the manufacturer’s recommendations. The QIAmp Fast DNA Stool kit (Qiagen, Hilden, Germany) was used to extract genomic DNA from faecal samples. Fragment size analyses were performed to obtained allele size quantification for the 14 microsatellites and the sex marker, by using a SeqStudio Genetic Analyzer (Applied Biosystems, Foster City, CA). Genetic profiles were determined using the Microsatellite Analysis module available on the Thermo Fisher cloud (https://apps.thermofisher.com/editor-web/#/app/app-microsatellites-web). References Breen, M., Jouquand, S., Renier, C., Mellersh, C.S., Hitte, C., Holmes, N.G., Chéron, A., Suter, N., Vignaux, F., Bristow, A.E., Priat, C., McCann, E., André, C., Boundy, S., Gitsham, P., Thomas, R., Bridge, W.L., Spriggs, H.F., Ryder, E.J., Curson, A., Sampson, J., Ostrander, E.A., Binns, M.M., Galibert, F., 2001. Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes. Genome Res. 11, 1784–1795. Fredholm, M., Winterø, A.K., 1995. Variation of short tandem repeats within and between species belonging to the Canidae family. Mamm. Genome Off. J. Int. Mamm. Genome Soc. 6, 11–18. Holmes, N.G., Dickens, H.F., Parker, H.L., Binns, M.M., Mellersh, C.S., Sampson, J., 1995. Eighteen canine microsatellites. Anim. Genet. 26, 132–133. Ichikawa, Y., Takahashi, Y., Tsumagari, S., Takeishi, M., Ishihama, K., Morita, M., Kanemaki, M., Minezawa, M., Takahashi, H., 2002. Identification and characterization of 40 dinucleotide microsatellites in the dog genome. Anim. Genet. 33, 400–401. Moore, M., Brown, K.S., Sacks, A., 2010. Thirty-one short red fox (Vulpes vulpes) microsatellite markers. Mol. Ecol. Resour. 10, 404–408. Ostrander, E.A., Sprague, G.F., Rine, J., 1993. Identification and characterization of dinucleotide repeat (CA)n markers for genetic mapping in dog. Genomics 16, 207–213.

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