Metabolomics of CD4+ T cells supplemented with NR vs. vehicle control
Metabolomics analysis of human CD4+ T cells isolated from healthy volunteers, differentiated to Th0 and Th17 and supplemented with 0.5mM nicotinamide riboside (NR) or vehicle control.
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For complete details, please refer to Han et al. (2023) Boosting NAD preferentially blunts TH17 inflammation via arginine biosynthesis and redox regulatory control in control and psoriasis subjects. In brief, human CD4+ T cells isolated from healthy volunteers were activated with plate-coated CD3 and CD28 for 3 days, differentiated to Th0 or Th17, and supplemented with 0.5mM NR or vehicle control. Metabolomics was performed by LC-MS. Metabolite abundance was normalized to protein content. SIMCA software (version 15; Umetrics) was used to perform orthogonal partial least squares discriminant analysis (OPLS-DA) and identify variable importance in prediction (VIP) metabolites to discriminate NR vs. Vehicle control. Univariate statistics were generated in MetaboAnalyst (https://www.metaboanalyst.ca/).