Mitophagy decreases in the peripheral vestibular system of aged C57BL/6J mice

Published: 10 March 2023| Version 1 | DOI: 10.17632/hv7pjssx7r.1


Figure 1. Locomotor performance of young and aged mice on rotarod test. Figure 2. Changes in mitochondrial DNA integrity (A), ATP content (B), and the expression of mitophagy-related genes (C) in the aged vestibular system. Figure 3. Changes in the expression of mitophagy-related and oxidative phosphorylation (OXPHOS) proteins in the aged vestibular system. Figure 4. Impaired mitophagy in the aged vestibular organs of C57BL/6J mice. Figure 5. Decreased Parkin and BNIP3 in the aged vestibular organs of C57BL/6J mice.


Steps to reproduce

Balance was assessed using the rotarod test. Mitochondrial DNA integrity, ATP content, and mitophagy-related genes and proteins were investigated by real-time PCR and western blot analysis. Quantification of mitophagosomes and mitophagolysosomes in the saccule, utricles, and crista ampullaris was performed using immunofluorescence imaging. Two mitophagy associated factors, Parkin and BNIP3, were investigated through immunohistochemical staining of the peripheral vestibular system.


Chosun University


Neuroscience, Otology


National Research Foundation of Korea