Three-dimensional differentiation of human pluripotent stem cell-derived neural precursor cells using tailored porous polymer scaffolds
Description
The data here represents the characterisation of human pluripotent stem cell-derived neural progenitor cells (hPSC-NPCs) maintained and differentiated three dimensionally within porous polymer scaffolds. The following raw and processed data is presented: - Viability of HDF51i-509-hNPCs (iPSC-NPCs) maintained within PEGDA and TMPTA scaffolds and on 2D TCPS quantified using the PrestoBlue assay and intensity measured by fluorescent plate reader. - Number of HDF51i-509-hNPs (iPSC-NPCs) maintained within PEGDA and TMPTA scaffolds and on 2D TCPS quantified using the PicoGreen dsDNA assay and intensity measured by fluorescent plate reader. - Expression of RNA relating to early neural markers PAX6, SOX1, TUBB3 and GFAP of both iPSC-hNPCs and H9-hNPCs (ESC-NPCs) maintained for 14 days within PEGDA and TMPTA scaffolds and on 2D TCPS quantified by RT-qPCR. - Expression of RNA relating to early neural markers PAX6, SOX1, TUBB3, MAP2, SYP and GFAP of both iPSC-hNPCs and H9-hNPCs (ESC-hNPCs) differentiated within PEGDA and TMPTA scaffolds and on 2D TCPS for 10, 19, 28 and 45 days quantified by RT-qPCR. - Calcium imaging video files relating to iPSC-hNPC and ESC-hNPC cultures differentiated for 49 days with PEGDA scaffolds and on 2D TCPS. Cultures were imaged for spontaneous activity, after the addition of glutamate as well as after the addition of a vehicle control. Raw video files and processed intensity data is presented here. - Storage and loss modulus data from strain, frequency and time sweep rheological experiments on PEGDA and TMPTA scaffolds.