Amaranthus tuberculatus, Amaranthus hybridus and Amaranthus retroflexus acetolactate synthase (ALS) gene, partial cds

Published: 25 October 2019| Version 1 | DOI: 10.17632/hxrvf9cpkx.1
Contributors:
Andrea Milani,
Laura Scarabel,
Maurizio Sattin

Description

This repository has been created as a partial fulfilment of the peer-review revision process of the paper “A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy” submitted to Pest Management Science. This repository contains all the electropherograms (ABI format chromatogram file, ab1) obtained by Sanger sequencing of Amaranthus spp plants survived to the field dose of thifensulfuron-methyl discussed in the paper named above (that was under revision at the time of the dataset creation). Three Amaranthus species were involved in the study (namely, Amaranthus tuberculatus, Amaranthus hybridus and Amaranthus retroflexus) and 12 resistant populations were described. Previously described primers(1) were used to amplify specific traits of acetolactate synthase (ALS) gene, where resistance-endowing mutation were previously observed: 3F/4R were used to amplify the region surrounding the position 376, whereas primers 2F/2R were used for position 574. Amplicons were purified and primers 3F or 2R were used for Sanger sequencing. Original ab1 files are herein reported, without modifications, except for sequence names. In order to facilitate data interpretation, the sequences have been renamed as: EPPOcode_population-code_Sample#_SequencingPrimer.ab1 EPPO(2) codes AMARE, AMACH and AMATU refer to Amaranthus retroflexus, Amaranthus hybridus and Amaranthus tuberculatus, respectively. Population codes are the same used in the paper. Sequences amplified with primer 2R should be reverse-complemented before reading. Bibliography: 1 Scarabel L, Varotto S, and Sattin M, A European biotype of Amaranthus retroflexus cross-resistant to ALS inhibitors and response to alternative herbicides, Weed Res 47:527–533 (2007). 2 EPPO, EPPO Global Database (available online), 2019. https://gd.eppo.int/ [accessed 10 July 2019].

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