A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation
Description
Figure 1: Western blot for indicated protein：1B, 1C, 1D, 1E, 1I, 1K. CBB staining： 1D Figure2：In vitro cleavage assay for indicated mRNAs: 2L EMSA showing the binding of DGCR8 to the stem-loop structure of indicated RNAs: 2K Figure3: Images of cell morphology during EB differentiation of WT and FLII ko cells: 3B. Western blot for indicated protein：3F Figure4: Western blot of indicated proteins: 4C, 4D, 4E, 4H, 4J, 4K, 4L Figure5: Western blot of indicated proteins: 5A, 5B, 5C, 5D, 5E, 5J Figure6: Western blot of indicated proteins: 6D, 6G Figure7: Microscopy images of immunofluorescence of mouse embryo: 7E Microscopy images of immunofluorescence spheroid derived from mESC: 7F, 7G,7H,7I FigureS1: Microscopy images of immunofluorescence of mESC for DGCR8/FLII location: S1C FigureS2: EMSA showing the binding of DGCR8 to the stem-loop structure of indicated RNAs: S2J, S2K Western blot of indicated proteins: S2N FigureS4：Western blot for indicated protein：S4B, S4D FigureS5：Western blot for indicated protein：S5D, S5E FigureS6：Western blot for indicated protein：S6C