Three-dimensional human alveolar stem cell culture models reveal infection response to SARS-CoV-2

Published: 12 October 2020| Version 1 | DOI: 10.17632/hzbrzdkcfr.1
Jeonghwan Youk,
taewoo kim,
Kelly Evans,
Young-Il Jeong,
Yongsuk Hur,
seonpyo hong,
Je Hyoung Kim,
Kijong Yi,
Su Yeon Kim,
Kwon Joong Na,
Thomas Bleazard,
Homin Kim,
Mick Fellows,
Krishnaa Mahbubani,
Kourosh Saeb-Parsy,
Seon Young Kim,
Young Tae Kim,
Gou Young Koh,
Byeong-sun Choi,
Young Seok Ju,
Joo-Hyeon Lee


The research developed human 3D alveolar culture which can be used to study SARS-CoV-2's infection mechanism. In short, We builtㅣlong-term, 3D culture models of hAT2 cells derived from primary human lung tissues in chemically defined culture condition . hAT2 cells are permissive to SARS-CoV-2 infection. Single cell RNA sequencing identififes immune proficient and deficienct populations of infected hAT2 cells. One SARS-CoV2 virus particle sufficiently infects a hAT2 cell from viral mutation. Supple_information is supplemental figures with legends. For figure S1, establishment and characterization of three-dimensional culture of human airway and alveolar type 2 cells. Figure S2 is Lactate dehydrogenase cytotoxicity assay of human 3D alveolar cultures and Vero cells Figure S3 is confocal imaging analysis of infected h3ACs. Figure S4 is transmission electron microscopy imaging of uninfected h3ACs. Figure S5 is transcriptome changes of the infected h3ACs. Figure S6 is single cell transcriptome profiling of infected h3ACs. Table S6 is clinical characteristics of tissue donors. Table S7 is list of quantitative PCR primers. Key_resource_table is the list of all reagents used in this study Supplemental tables is largely divided into 4 categories. Table S1 and S3 TPM of h3AC, h3BC are the RNA sequencing TPM results of infected and uninfected h3AC and h3BC. Table S2. is List of differentially upregulated genes in SARS-CoV-2 infected human lung, h3AC, Vero cell line, Calu-3 cell line, and Caco-2 cell lines. Table S4. is Single base substitution list is made from RNA sequencing of h3AC and h3BC. Table S5 is DEGs were also made from single cell RNA sequencing.