Myc-interacting Zinc Finger Protein 1 Suppresses Liver Tumorigenesis by Restricting Hepatocyte-driven Inflammation Independently of Its Transcriptional Activity
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All raw data of Western blots and mass spectrometry of the paper titled "Myc-interacting Zinc Finger Protein 1 Suppresses Liver Tumorigenesis by Restricting Hepatocyte-driven Inflammation Independently of Its Transcriptional Activity "
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WB: Cells and liver samples were homogenized and ruptured using ice-cold RIPA buffer containing fresh protease and phosphatase inhibitors (Beyotime, Nantong, China) and the protein concentration was measured using the BCA assay (Beyotime, Nantong, China). After denaturation, protein samples were subjected to SDS-PAGE. When multiple target proteins were analyzed in the same experiment, not all the target proteins could be analyzed by a single immunoblot. Thus, the aliquots of the same experimental sample were analyzed by separated SDS-gel electrophoreses with individual loading controls, followed by separated immunoblotting analyses. The data of multiple immunoblots along with a representative loading control were placed in a signal figure panel. Blots were visualized with an enhanced chemiluminescence detection kit (Pierce, Thermo Scientific, USA) and the ChemiDocTM MP Imaging System (Bio-Rad). Mass spectrometry: Proteins from cell lysis (Huh7-Miz1His and SMMC-7721-Miz1His) were immunoprecipitated with anti-His antibody, separated by SDS-PAGE, and finally visualized with coomassie brilliant blue staining. Protein in-gel digestion and nano-HPLC MS/MS were carried out. The acquired MS/MS spectra were searched against the National Center for Biotechnology Information nonredundant protein sequence database.