Comparison of isolation methods using commercially available kits for obtaining extracellular vesicles from cow milk (supplementary files)

Published: 11-03-2021| Version 1 | DOI: 10.17632/j38whndhhy.1
Contributors:
Mai Morozumi,
Takashi Shimizu,
Yasuhiro Takeda

Description

Supplementary Figure 1. Measurement of EV counts and protein concentrations in each fraction from qEV. (A) Combination of centrifugation-based casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (B) Combination acetic acid precipitation-mediated casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (C) Combination of EDTA precipitation-mediated casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). Supplementary Figure 2. Measurement of EV counts and protein concentration in each fraction from EVSecond L70. (A) Combination of centrifugation-mediated casein removal and EVSecond L70. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3); (B) Combination of acetic acid precipitation-mediated casein removal and an EVSecond L70. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (C) Combination of EDTA precipitation-mediated casein removal and EVSecond L70. Black circles EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3).

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