Data of Baicalin

Published: 30-06-2020| Version 3 | DOI: 10.17632/jm3fjvjyf2.3
Contributor:
Zhang Ling

Description

Results 1. Effects of Baicalin on cell viability and LDH production Note: RAW264.7 cells viability——RAW细菌感染细胞活性 H9 cells viability——H9细菌感染细胞活性 1——control group;2——S. typhimurium group;3,4,5——S. typhimurium + Baicalin (25, 50, 100 μmol/L) group; 6——100 μmol/L Baicalin group Note: LDH of RAW264 cells——LDHRAW264 LDH of H9 cells cells——LDHH9 1——control group;2——S. typhimurium group;3,4,5——S. typhimurium + Baicalin (25, 50, 100 μmol/L) group; 6——100 μmol/L Baicalin group 2. Effects of Baicalin on bactericidal effect Note: Bactericidal effect——rateofkilling 1——S. typhimurium group;2,3,4——S. typhimurium + Baicalin (25, 50, 100 μmol/L) group. 3. Effects of Baicalin on inflammatory cytokines and oxidative stress induced by S. typhimurium Note: The levels of TNF-α——TNFz;The levels of IL-1β——IL1z;The levels of IL-6——IL6z;The levels of IL-8——IL8z 1——control group;2——S. typhimurium group;3,4,5——S. typhimurium + Baicalin (25, 50, 100 μmol/L) group; 6——100 μmol/L Baicalin group ROS production——ROS 1——control group;2——S. typhimurium group;3,4,5——S. typhimurium + Baicalin (25, 50, 100 μmol/L) group; 6——100 μmol/L Baicalin group 4. Baicalin mediated the autophagy and activation of TLR4 pathway induced by S. typhimurium Note: LC3 immuno-positive cells——荧光百分率 1——control group;2——S. typhimurium group;3——S. typhimurium + Baicalin (100 μmol/L) group; 4——100 μmol/L Baicalin group the expression of LC3-II/I——LC3;the expression of Beclin 1——Beclin 1 1——control group;2——S. typhimurium group;3——S. typhimurium + Baicalin (100 μmol/L) group; 4——100 μmol/L Baicalin group the expression of TLR4, p-p38/p38, p-JNK/JNK, p-ERK/ERK——TLR4, p38, JNK, ERK 1——control group;2——S. typhimurium group;3——S. typhimurium + Baicalin (100 μmol/L) group; 4——100 μmol/L Baicalin group 5. Effects of Baicalin on S. typhimurium-induced autophagy and activation of TLR4 pathway in TLR4 overexpression Note: the expression of TLR4——pcDNATLR4 1——pcDNA3.1(+) group;2——pcDNA3.1-TLR4 group; the expression of LC3, Beclin1, TLR4, p-p38/p38, p-JNK/JNK, p-ERK/ERK——LC32, Beclin12, TLR42, p382, JNK2, ERK2 1——pcDNA3.1(+)-S. typhimurium group;2——pcDNA3.1(+)-S. typhimurium + Baicalin group; 3—— pcDNA3.1-TLR4 -S. typhimurium group;4——pcDNA3.1-TLR4 -S. typhimurium + Baicalin group

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