Synergistic Effects of Superdose Phytase and Acidifier Supplementation on Performance, Nutrient Utilization, and Blood Profile in Broiler Chickens Fed Phosphorus-Adequate or Reduced Diets

Published: 1 June 2026| Version 1 | DOI: 10.17632/jnxfd6ndrz.1
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A total of 400-day-old broiler chickens of the Ross 308 strain were used in a completely randomized design, with 5 dietary treatments and 4 replications, each containing 20 birds. The treatments were as follows: 1. Control (basal diet without additives); 2. Control + Phytase: (PHY; 2000 FTU/kg); 3. Control + acidifier (ACI; 3 gr/ kg); 4. Control + Phytase + acidifier (PHAC), and 5. Low-phosphorus diet + Phytase + Acidifier (LPHAC). In total period (1–42 days), feed conversion ratio (FCR) was significantly improved in birds receiving PHAC and LPHAC diets compared to those fed the control diet supplemented only with ACI diet (P<0.05). Additionally, ALP enzyme increased in birds fed with ACI and PHAC diets, compared to chicks fed control with LPHAC diet (P<0.05). Nitrogen level of birds decreased when fed control with LPHAC diet, compared to birds fed on the control diet (P<0.05). Based on the results, supplementation of phytase enzyme and acidifier, especially when combined with reduced dietary phosphorus, improved early growth performance, feed efficiency, and intestinal morphology in broilers. Data Availability: Raw data for growth performance, blood profileare and litter characteristics available from the corresponding author. All processed datasets and analytical outputs will be provided upon reasonable request. Declaration of competing interest: We declare that we have no financial and personal relationships with other people or organizations that can in appropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper

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Poultry, Industrial Phytases Application

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