Adult Females with Barium (Ba) and Strontium (Sr) Data

Published: 17 August 2019| Version 1 | DOI: 10.17632/jthr5mptvd.1
Mack Frantz


Research article: Epigenetic response of Louisiana Waterthrush (Parkesia motacilla) related to shale gas development Journal: Ibis Citation: M. W. Frantz, P. B. Wood, S. Latta, and A. Welsh. 2019. Epigenetic response of Louisiana Waterthrush (Parkesia motacilla) related to shale gas development. Ibis In Review. Contact information: Mack W. Frantz;; West Virginia Cooperative Fish and Wildlife Research Unit, Davis College, School of Natural Resources, West Virginia University, P.O. Box 6125, Morgantown, WV, USA Analysis description: If TerrGas or TerrRunoff was statistically significant for adult females, the restriction sites driving the overall differential methylation were used to correlate the total amount of methylation across restriction sites to Barium (Ba) and Stontium (Sr) heavy concentrations bioaccumulated in waterthrush feathers. Otherwise, all restriction sites were used. The test was limited to waterthrush sampled in 2013 that had both blood samples and Ba and Sr feather data from Latta et al. (2015). A Box-Cox transformation was performed on Ba and Sr data (in ppm) to approach normality using R package car (Fox et al. 2015). We used the transformed Ba and Sr data to conduct a Pearson correlation of the total amount of methylation across restriction sites to Ba and Sr concentrations using R package psych (Revelle 2017) in R (R Core Team 2014). See research article for additional information regarding methdology, terms, and data significance. Data description: Adult females with Strontium (Sr) and Barium (Ba) data from 2013 have Box-Cox transformed ppm values displayed as used in the Pearson correlations. Columns A and B have heavy metal values used in analysis. Column C indicates the number of methylated restriction sites across all restriction sites (n=100). Four adult female recaptures had Ba and Sr data described. Research article relationship: Data referenced in Table 3 and displayed in Fig. 1. References: Fox, J., Weisberg, S., Adler, D., Bates, D., Baud-Bovy, G., Ellison, S., Firth, D., Friendly, M., Gorjanc, G., Graves, S., Heiberger, R., Laboissiere, R., Monette, G., Murdoch, D., Nilsson, H., Ogle, D., Ripley, B., Venables, W., Winsemius, D., Zeileis, A., & R-Core. 2017. Package ‘car’. Version 2.1-5. (accessed 2017 July 19). Latta, S.C., Marshall, L.C., Frantz, M.W., & Toms, J. 2015. Evidence from two shale regions that a riparian songbird accumulates metals associated with hydraulic fracturing. Ecosphere 6: 144. R Development Core Team. 2014. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Revelle, W. 2017. Package ‘psych’. Version 1.7.5. (accessed 2017 July 19).



West Virginia University


Ornithology, Heavy Metal, DNA Methylation, Epigenetics